1jk4
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DES 1-6 BOVINE NEUROPHYSIN II COMPLEX WITH VASOPRESSIN
OverviewOverview
The structures of des 1-6 bovine neurophysin-II in the unliganded state, and as its complex with lysine vasopressin were determined, crystallographically at resolutions of 2.4 A and 2.3 A, respectively. The, structure of the protein component of the vasopressin complex was, with, some local differences, similar to that determined earlier of the, full-length protein complexed with oxytocin, but relatively large, differences, probably intrinsic to the hormones, were observed between the, structures of bound oxytocin and bound vasopressin at Gln 4. The structure, of the unliganded protein is the first structure of an unliganded, neurophysin. Comparison with the liganded state indicated significant, binding-induced conformational changes that were the largest in the loop, region comprising residues 50-58 and in the 7-10 region. A subtle, binding-induced tightening of the subunit interface of the dimer also was, shown, consistent with a role for interface changes in neurophysin, allosteric mechanism, but one that is probably not predominant. Interface, changes are suggested to be communicated from the binding site through the, strands of beta-sheet that connect these two regions, in part with, mediation by Gly 23. Comparison of unliganded and liganded states, additionally reveals that the binding site for the hormone alpha-amino, group is largely preformed and accessible in the unliganded state, suggesting that it represents the initial site of hormone protein, recognition. The potential molecular basis for its thermodynamic, contribution to binding is discussed.
About this StructureAbout this Structure
1JK4 is a Protein complex structure of sequences from Bos taurus with CD as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structures of an unliganded neurophysin and its vasopressin complex: implications for binding and allosteric mechanisms., Wu CK, Hu B, Rose JP, Liu ZJ, Nguyen TL, Zheng C, Breslow E, Wang BC, Protein Sci. 2001 Sep;10(9):1869-80. PMID:11514677
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