1jjh
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E2 DNA-binding Domain from Bovine Papillomavirus Type 1
OverviewOverview
The 2.5 A crystal structures of the DNA-binding domain of the E2 protein, from bovine papillomavirus strain 1 and its complex with DNA are, presented. E2 is a transcriptional regulatory protein that is also, involved in viral DNA replication. It is the structural prototype for a, novel class of DNA-binding proteins: dimeric beta-barrels with surface, alpha-helices that serve as recognition helices. These helices contain the, amino-acid residues involved in sequence-specifying interactions. The E2, proteins from different papillomavirus strains recognize and bind to the, same consensus 12 base-pair DNA sequence. However, recent evidence from, solution studies points to differences in the mechanisms by which E2 from, the related viral strains bovine papillomavirus-1 and human, papillomavirus-16 discriminate between DNA targets based on non-contacted, nucleotide sequences. This report provides evidence that sequence-specific, DNA-binding is accompanied by a rearrangement of protein subunits and, deformation of the DNA. These results suggest that, along with DNA, sequence-dependent conformational properties, protein subunit orientation, plays a significant role in the mechanisms of target selection utilized by, E2.
About this StructureAbout this Structure
1JJH is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
ReferenceReference
Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein., Hegde RS, Wang AF, Kim SS, Schapira M, J Mol Biol. 1998 Mar 6;276(4):797-808. PMID:9500927
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