1j5c
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SOLUTION STRUCTURE OF OXIDIZED PARAMAGNETIC CU(II) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803
OverviewOverview
The NMR solution structure of oxidized plastocyanin from the, cyanobacterium Synechocystis PCC6803 is here reported. The protein, contains paramagnetic copper(II), whose electronic relaxation times are, quite unfavorable for NMR solution studies. The structure has been solved, on the basis of 1041 meaningful NOESY cross-peaks, 18 1D NOEs, 26 T(1), values, 96 dihedral angle constraints, and 18 H-bonds. The detection of, broad hyperfine-shifted signals and their full assignment allowed the, identification of the copper(II) ligands and the determination of the, Cu-S-C-H dihedral angle for the coordinated cysteine. The global, root-mean-square deviation from the mean structure for the solution, structure family is 0.72 +/- 0.14 and 1.16 +/- 0.17 A for backbone and, heavy atoms, respectively. The structure is overall quite satisfactory and, represents a breakthrough, in that it includes paramagnetic copper, proteins among the metalloproteins for which solution structures can be, afforded. The comparison with the available X-ray structure of a triple, mutant is also performed.
About this StructureAbout this Structure
1J5C is a Single protein structure of sequence from Synechocystis sp. with CU as ligand. This structure superseeds the now removed PDB entry 1I0W. Full crystallographic information is available from OCA.
ReferenceReference
The first solution structure of a paramagnetic copper(II) protein: the case of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803., Bertini I, Ciurli S, Dikiy A, Fernandez CO, Luchinat C, Safarov N, Shumilin S, Vila AJ, J Am Chem Soc. 2001 Mar 14;123(10):2405-13. PMID:11456890
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