1j35
|
Crystal Structure of Ca(II)-bound Gla Domain of Factor IX Complexed with Binding Protein
OverviewOverview
Factor IX is an indispensable protein required in the blood coagulation, cascade. It binds to the surface of phospholipid membrane by means of a, gamma-carboxyglutamic acid (Gla) domain situated at the N terminus., Recently, we showed that physiological concentrations of Mg2+ ions affect, the native conformation of the Gla domain and in doing so augment the, biological activity of factor IXa and binding affinity with its binding, protein even in the presence of Ca2+ ions. Here we report on the crystal, structures of the Mg2+/Ca2+-bound and Ca2+-bound (Mg2+-free) factor IX Gla, domain (IXGD1-46) in complex with its binding protein (IX-bp) at 1.55 and, 1.80 A resolutions, respectively. Three Mg2+ and five Ca2+ ions were bound, in the Mg2+/Ca2+-bound IXGD1-46, and the Mg2+ ions were replaced by Ca2+, ions in Mg2+-free IXGD1-46. Comparison of Mg2+/Ca2+-bound with Ca2+-bound, structures of the complexes showed that Mg2+ ion, which formed a bridge, between IXGD1-46 and IX-bp, forced IXGD1-46 to rotate 4 degrees relative, to IX-bp and hence might be the cause of a more tight interaction between, the molecules than in the case of the Mg2+-free structure. The results, clearly suggest that Mg2+ ions are required to maintain native, conformation and in vivo function of factor IX Gla domain during blood, coagulation.
About this StructureAbout this Structure
1J35 is a Protein complex structure of sequences from Bos taurus and Trimeresurus flavoviridis with CA as ligand. Active as Coagulation factor IXa, with EC number 3.4.21.22 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Mg2+- and Ca2+-bound Gla domain of factor IX complexed with binding protein., Shikamoto Y, Morita T, Fujimoto Z, Mizuno H, J Biol Chem. 2003 Jun 27;278(26):24090-4. Epub 2003 Apr 14. PMID:12695512
Page seeded by OCA on Tue Nov 20 17:53:46 2007