1j09
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Crystal structure of Thermus thermophilus glutamyl-tRNA synthetase complexed with ATP and Glu
OverviewOverview
Aminoacyl-tRNA synthetases catalyze the formation of an aminoacyl-AMP from, an amino acid and ATP, prior to the aminoacyl transfer to tRNA. A subset, of aminoacyl-tRNA synthetases, including glutamyl-tRNA synthetase (GluRS), have a regulation mechanism to avoid aminoacyl-AMP formation in the, absence of tRNA. In this study, we determined the crystal structure of the, 'non-productive' complex of Thermus thermophilus GluRS, ATP and, L-glutamate, together with those of the GluRS.ATP, GluRS.tRNA.ATP and, GluRS.tRNA.GoA (a glutamyl-AMP analog) complexes. In the absence of, tRNA(Glu), ATP is accommodated in a 'non-productive' subsite within the, ATP-binding site, so that the ATP alpha-phosphate and the glutamate, alpha-carboxyl groups in GluRS. ATP.Glu are too far from each other (6.2, A) to react. In contrast, the ATP-binding mode in GluRS.tRNA. ATP is, dramatically different from those in GluRS.ATP.Glu and GluRS.ATP, but, corresponds to the AMP moiety binding mode in GluRS.tRNA.GoA (the, 'productive' subsite). Therefore, tRNA binding to GluRS switches the, ATP-binding mode. The interactions of the three tRNA(Glu) regions with, GluRS cause conformational changes around the ATP-binding site, and allow, ATP to bind to the 'productive' subsite.
About this StructureAbout this Structure
1J09 is a Single protein structure of sequence from Thermus thermophilus with MG, GLU and ATP as ligands. Active as Glutamate--tRNA ligase, with EC number 6.1.1.17 Full crystallographic information is available from OCA.
ReferenceReference
ATP binding by glutamyl-tRNA synthetase is switched to the productive mode by tRNA binding., Sekine S, Nureki O, Dubois DY, Bernier S, Chenevert R, Lapointe J, Vassylyev DG, Yokoyama S, EMBO J. 2003 Feb 3;22(3):676-88. PMID:12554668
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- Glutamate--tRNA ligase
- Single protein
- Thermus thermophilus
- Bernier, S.
- Chenevert, R.
- Dubois, D.Y.
- Lapointe, J.
- Nureki, O.
- RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.
- Sekine, S.
- Vassylyev, D.G.
- Yokoyama, S.
- ATP
- GLU
- MG
- Glurs-atp-glu complex
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomics