1itc
Beta-Amylase from Bacillus cereus var. mycoides Complexed with Maltopentaose
|
OverviewOverview
The X-ray crystal structure of a catalytic site mutant of beta-amylase, E172A (Glu172 --> Ala), from Bacillus cereus var. mycoides complexed with, a substrate, maltopentaose (G5), and the wild-type enzyme complexed with, maltose were determined at 2.1 and 2.0 A resolution, respectively. Clear, and continuous density corresponding to G5 was observed in the active site, of E172A, and thus, the substrate, G5, was not hydrolyzed. All glucose, residues adopted a relaxed (4)C(1) conformation, and the conformation of, the maltose unit for Glc2 and Glc3 was much different from those of other, maltose units, where each glucose residue of G5 is named Glc1-Glc5 (Glc1, is at the nonreducing end). A water molecule was observed 3.3 A from the, C1 atom of Glc2, and 3.0 A apart from the OE1 atom of Glu367 which acts as, a general base. In the wild-type enzyme-maltose complex, two maltose, molecules bind at subsites -2 and -1 and at subsites +1 and +2 in tandem., The conformation of the maltose molecules was similar to that of the, condensation product of soybean beta-amylase, but differed from that of G5, in E172A. When the substrate flips between Glc2 and Glc3, the, conformational energy of the maltose unit was calculated to be 20 kcal/mol, higher than that of the cis conformation by MM3. We suggest that, beta-amylase destabilizes the bond that is to be broken in the ES complex, decreasing the activation energy, DeltaG(++), which is the difference in, free energy between this state and the transition state.
About this StructureAbout this Structure
1ITC is a Single protein structure of sequence from Bacillus cereus with GLC, CA, SO4 and ACY as ligands. Active as Beta-amylase, with EC number 3.2.1.2 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a catalytic site mutant of beta-amylase from Bacillus cereus var. mycoides cocrystallized with maltopentaose., Miyake H, Kurisu G, Kusunoki M, Nishimura S, Kitamura S, Nitta Y, Biochemistry. 2003 May 20;42(19):5574-81. PMID:12741813
Page seeded by OCA on Tue Nov 20 17:39:16 2007