1ih7
High-Resolution Structure of Apo RB69 DNA Polymerase
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OverviewOverview
We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase, with primer-template DNA and dTTP, capturing the step just before primer, extension. This ternary complex structure in the human DNA polymerase, alpha family shows a 60 degrees rotation of the fingers domain relative to, the apo-protein structure, similar to the fingers movement in pol I family, polymerases. Minor groove interactions near the primer 3' terminus suggest, a common fidelity mechanism for pol I and pol alpha family polymerases., The duplex product DNA orientation differs by 40 degrees between the, polymerizing mode and editing mode structures. The role of the thumb in, this DNA motion provides a model for editing in the pol alpha family.
About this StructureAbout this Structure
1IH7 is a Single protein structure of sequence from Enterobacteria phage rb18 with K and GMP as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
ReferenceReference
Structure of the replicating complex of a pol alpha family DNA polymerase., Franklin MC, Wang J, Steitz TA, Cell. 2001 Jun 1;105(5):657-67. PMID:11389835
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