1gox
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REFINED STRUCTURE OF SPINACH GLYCOLATE OXIDASE AT 2 ANGSTROMS RESOLUTION
OverviewOverview
The amino acid sequence of glycolate oxidase from spinach has been fitted, to an electron density map of 2.0 A nominal resolution and the structure, has been refined using the restrained parameter least-squares refinement, of Hendrickson and Konnert. A final crystallographic R-factor of 18.9% was, obtained for 32,888 independent reflections from 5.5 to 2 A resolution., The geometry of the model, consisting of 350 amino acid residues, the, cofactor flavin mononucleotide and 298 solvent molecules, is close to, ideal with root-mean-square deviations of 0.015 A in bond lengths and 2.6, degrees in bond angles. The expected trimodal distribution with preference, for staggered conformation is obtained for the side-chain chi 1-angles., The core of the subunit is built up from the eight beta-strands in the, beta/alpha-barrel. This core consists of two hydrophobic layers. One in, the center is made up of residues pointing in from the beta-strands, towards the barrel axis and the second, consisting of two segments of, residues, pointing out from the beta-strands towards the eight, alpha-helices of the barrel and pointing from the helices towards the, strands. The hydrogen bond pattern for the beta-strands in the, beta/alpha-barrel is described. There are a number of residues with, 3(10)-helix conformation, in particular there is one left-handed helix., The ordered solvent molecules are organized mainly in clusters. The, average isotropic temperature factor is quite high, 27.1 A2, perhaps a, reflection of the high solvent content in the crystal. The octameric, glycolate oxidase molecule, which has 422 symmetry, makes strong, interactions around the 4-fold axis forming a tight tetramer, but only, weak interactions between the two tetramers forming the octamer.
About this StructureAbout this Structure
1GOX is a Single protein structure of sequence from Spinacia oleracea with ACE and FMN as ligands. Active as (S)-2-hydroxy-acid oxidase, with EC number 1.1.3.15 Full crystallographic information is available from OCA.
ReferenceReference
Refined structure of spinach glycolate oxidase at 2 A resolution., Lindqvist Y, J Mol Biol. 1989 Sep 5;209(1):151-66. PMID:2681790
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