1gbq
SOLUTION NMR STRUCTURE OF THE GRB2 N-TERMINAL SH3 DOMAIN COMPLEXED WITH A TEN-RESIDUE PEPTIDE DERIVED FROM SOS DIRECT REFINEMENT AGAINST NOES, J-COUPLINGS, AND 1H AND 13C CHEMICAL SHIFTS, MINIMIZED AVERAGE STRUCTURE
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OverviewOverview
Refined ensembles of solution structures have been calculated for the, N-terminal SH3 domain of Grb2 (N-SH3) complexed with the ac-VPPPVPPRRR-nh2, peptide derived from residues 1135 to 1144 of the mouse SOS-1 sequence., NMR spectra obtained from different combinations of both 13C-15N-labeled, and unlabeled N-SH3 and SOS peptide fragment were used to obtain, stereo-assignments for pro-chiral groups of the peptide, angle restraints, via heteronuclear coupling constants, and complete 1H, 13C, and 15N, resonance assignments for both molecules. One ensemble of structures was, calculated using conventional methods while a second ensemble was, generated by including additional direct refinements against both 1H and, 13C(alpha)/13C(beta) chemical shifts. In both ensembles, the, protein:peptide interface is highly resolved, reflecting the inclusion of, 110 inter-molecular nuclear Overhauser enhancement (NOE) distance, restraints. The first and second peptide-binding sub-sites of N-SH3, interact with structurally well-defined portions of the peptide. These, interactions include hydrogen bonds and extensive hydrophobic contacts. In, the third highly acidic sub-site, the conformation of the peptide Arg8, side-chain is partially ordered by a set of NOE restraints to the Trp36, ring protons. Overall, several lines of evidence point to dynamical, averaging of peptide and N-SH3 side-chain conformations in the third, subsite. These conformations are characterized by transient charge, stabilized hydrogen bond interactions between the peptide arginine, side-chain hydrogen bond donors and either single, or possibly multiple, acceptor(s) in the third peptide-binding sub-site.
About this StructureAbout this Structure
1GBQ is a Protein complex structure of sequences from Mus musculus with ACE and NH2 as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of the Grb2 N-terminal SH3 domain complexed with a ten-residue peptide derived from SOS: direct refinement against NOEs, J-couplings and 1H and 13C chemical shifts., Wittekind M, Mapelli C, Lee V, Goldfarb V, Friedrichs MS, Meyers CA, Mueller L, J Mol Biol. 1997 Apr 11;267(4):933-52. PMID:9135122
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