1oah
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CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774: THE RELEVANCE OF THE TWO CALCIUM SITES IN THE STRUCTURE OF THE CATALYTIC SUBUNIT (NRFA).
OverviewOverview
The gene encoding cytochrome c nitrite reductase (NrfA) from Desulfovibrio, desulfuricans ATCC 27774 was sequenced and the crystal structure of the, enzyme was determined to 2.3-A resolution. In comparison with homologous, structures, it presents structural differences mainly located at the, regions surrounding the putative substrate inlet and product outlet, and, includes a well defined second calcium site with octahedral geometry, coordinated to propionates of hemes 3 and 4, and caged by a loop, non-existent in the previous structures. The highly negative electrostatic, potential in the environment around hemes 3 and 4 suggests that the main, role of this calcium ion may not be electrostatic but structural, namely, in the stabilization of the conformation of the additional loop that ... [(full description)]
About this StructureAbout this Structure
1OAH is a [Single protein] structure of sequence from [Desulfovibrio desulfuricans] with CA, CL, ZN and HEM as [ligands]. Active as [[1]], with EC number [1.7.2.2]. Full crystallographic information is available from [OCA].
ReferenceReference
Cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA)., Cunha CA, Macieira S, Dias JM, Almeida G, Goncalves LL, Costa C, Lampreia J, Huber R, Moura JJ, Moura I, Romao MJ, J Biol Chem. 2003 May 9;278(19):17455-65. Epub 2003 Mar 4. PMID:12618432
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