1g8h
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ATP SULFURYLASE FROM S. CEREVISIAE: THE TERNARY PRODUCT COMPLEX WITH APS AND PPI
OverviewOverview
ATP sulfurylases (ATPSs) are ubiquitous enzymes that catalyse the primary, step of intracellular sulfate activation: the reaction of inorganic, sulfate with ATP to form adenosine-5'-phosphosulfate (APS) and, pyrophosphate (PPi). With the crystal structure of ATPS from the yeast, Saccharomyces cerevisiae, we have solved the first structure of a member, of the ATP sulfurylase family. We have analysed the crystal structure of, the native enzyme at 1.95 Angstroms resolution using multiple isomorphous, replacement (MIR) and, subsequently, the ternary enzyme product complex, with APS and PPi bound to the active site. The enzyme consists of six, identical subunits arranged in two stacked rings in a D:3 symmetric, assembly. Nucleotide binding causes significant conformational changes, which lead to a rigid body structural displacement of domains III and IV, of the ATPS monomer. Despite having similar folds and active site design, examination of the active site of ATPS and comparison with known, structures of related nucleotidylyl transferases reveal a novel ATP, binding mode that is peculiar to ATP sulfurylases.
About this StructureAbout this Structure
1G8H is a Single protein structure of sequence from Saccharomyces cerevisiae with CD, CA, NA, MG, ADX, POP and ACY as ligands. Active as Sulfate adenylyltransferase, with EC number 2.7.7.4 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of ATP sulfurylase from Saccharomyces cerevisiae, a key enzyme in sulfate activation., Ullrich TC, Blaesse M, Huber R, EMBO J. 2001 Feb 1;20(3):316-29. PMID:11157739
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