1g6t
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STRUCTURE OF EPSP SYNTHASE LIGANDED WITH SHIKIMATE-3-PHOSPHATE
OverviewOverview
Biosynthesis of aromatic amino acids in plants, many bacteria, and, microbes relies on the enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP), synthase, a prime target for drugs and herbicides. We have identified the, interaction of EPSP synthase with one of its two substrates (shikimate, 3-phosphate) and with the widely used herbicide glyphosate by x-ray, crystallography. The two-domain enzyme closes on ligand binding, thereby, forming the active site in the interdomain cleft. Glyphosate appears to, occupy the binding site of the second substrate of EPSP synthase, (phosphoenol pyruvate), mimicking an intermediate state of the ternary, enzyme.substrates complex. The elucidation of the active site of EPSP, synthase and especially of the binding pattern of glyphosate provides a, valuable roadmap for engineering new herbicides and herbicide-resistant, crops, as well as new antibiotic and antiparasitic drugs.
About this StructureAbout this Structure
1G6T is a Single protein structure of sequence from Escherichia coli with PO4, S3P and FMT as ligands. Active as 3-phosphoshikimate 1-carboxyvinyltransferase, with EC number 2.5.1.19 Full crystallographic information is available from OCA.
ReferenceReference
Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail., Schonbrunn E, Eschenburg S, Shuttleworth WA, Schloss JV, Amrhein N, Evans JN, Kabsch W, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1376-80. PMID:11171958
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