1g3k

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Revision as of 16:33, 20 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1g3k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g3k, resolution 1.90Å" /> '''CRYSTAL STRUCTURE OF...)
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File:1g3k.jpg


1g3k, resolution 1.90Å

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CRYSTAL STRUCTURE OF THE H. INFLUENZAE PROTEASE HSLV AT 1.9 A RESOLUTION

OverviewOverview

HslUV is a "prokaryotic proteasome" composed of the HslV protease and the, HslU ATPase, a chaperone of the Clp/Hsp100 family. The 3.4 A crystal, structure of an HslUV complex is presented here. Two hexameric ATP binding, rings of HslU bind intimately to opposite sides of the HslV protease; the, HslU "intermediate domains" extend outward from the complex. The solution, structure of HslUV, derived from small angle X-ray scattering data under, conditions where the complex is assembled and active, agrees with this, crystallographic structure. When the complex forms, the carboxy-terminal, helices of HslU distend and bind between subunits of HslV, and the apical, helices of HslV shift substantially, transmitting a conformational change, to the active site region of the protease.

About this StructureAbout this Structure

1G3K is a Single protein structure of sequence from Haemophilus influenzae with NA as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Crystal and solution structures of an HslUV protease-chaperone complex., Sousa MC, Trame CB, Tsuruta H, Wilbanks SM, Reddy VS, McKay DB, Cell. 2000 Nov 10;103(4):633-43. PMID:11106733

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