1fxw
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CRYSTAL STRUCTURE OF THE RECOMBINANT ALPHA1/ALPHA2 CATALYTIC HETERODIMER OF BOVINE BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE IB.
OverviewOverview
The intracellular form of mammalian platelet activating factor, acetylhydrolase found in brain (PAF-AH Ib) is thought to play a critical, role in control in neuronal migration during cortex development. This, oligomeric complex consists of a homodimer of the 45 kDa (beta) LIS1, protein, the product of the causative gene for type I lissencephaly, and, depending on the developmental stage and species, one of three possible, pairs of two homologous approximately 26 kDa alpha-subunits, which harbor, all of the catalytic activity. The exact composition of this complex, depends on the expression patterns of the alpha(1) and alpha(2) genes, exhibiting tissue specificity and developmental control. All three, possible dimers (alpha(1)/alpha(1), alpha(1)/alpha(2) and, alpha(2)/alpha(2)) were identified in tissues. The alpha(1)/alpha(2), heterodimer is thought to play an important role in fetal brain. The, structure of the alpha(1)/alpha(1) homodimer was solved earlier in our, laboratory at 1.7 A. We report here the preparation of recombinant, alpha(1)/alpha(2) heterodimers using a specially constructed bi-cistronic, expression vector. The approach may be useful in studies of other systems, where pure heterodimers of recombinant proteins are required. The, alpha(1)/alpha(2) dimer has been crystallized and its structure was solved, at 2.1 A resolution by molecular replacement. These results set the stage, for a detailed characterization of the PAF-AH Ib complex.
About this StructureAbout this Structure
1FXW is a Protein complex structure of sequences from Bos taurus with CA as ligand. Active as 1-alkyl-2-acetylglycerophosphocholine esterase, with EC number 3.1.1.47 Full crystallographic information is available from OCA.
ReferenceReference
Preparation and crystal structure of the recombinant alpha(1)/alpha(2) catalytic heterodimer of bovine brain platelet-activating factor acetylhydrolase Ib., Sheffield PJ, McMullen TW, Li J, Ho YS, Garrard SM, Derewenda U, Derewenda ZS, Protein Eng. 2001 Jul;14(7):513-9. PMID:11522926
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