1fw4

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Revision as of 16:14, 20 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1fw4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fw4, resolution 1.7Å" /> '''CRYSTAL STRUCTURE OF ...)
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File:1fw4.gif


1fw4, resolution 1.7Å

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CRYSTAL STRUCTURE OF E. COLI FRAGMENT TR2C FROM CALMODULIN TO 1.7 A RESOLUTION

OverviewOverview

Fragment TR2C is the C-terminal part of the calcium-binding protein, calmodulin, including residues 78-148. The crystal structure of TR2C was, solved by molecular replacement and refined to a conventional R value of, 21.8% (R(free) = 22.0%), using all data in the resolution range 20.0-1.7, A. This study shows that the secondary structure of TR2C, a pair of, EF-hand motifs with two calcium-binding sites, is similar to the, corresponding motifs in intact calmodulin. However, it also indicates that, the N-terminus of helix E is closer to the C-terminus of helix H in TR2C, than in the intact protein and that the loop connecting the EF-hands shows, different conformations in the two structures. The crystal structure of, TR2C was further found to be similar to the set of NMR structures of this, fragment, although some pronounced differences exist.

About this StructureAbout this Structure

1FW4 is a Single protein structure of sequence from Bos taurus with CA as ligand. This structure superseeds the now removed PDB entry 1TRC. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Escherichia coli fragment TR2C from calmodulin to 1.7 A resolution., Olsson LL, Sjolin L, Acta Crystallogr D Biol Crystallogr. 2001 May;57(Pt 5):664-9. Epub 2001, Apr 24. PMID:11320306

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