1fts
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SIGNAL RECOGNITION PARTICLE RECEPTOR FROM E. COLI
OverviewOverview
FtsY is the docking protein or SR alpha homologue in E. coli. It is, involved in targeting secretory proteins to the cytoplasmic membrane by, interacting with the signal recognition particle, controlled by guanosine, 5'-triphosphate. Two different constructs have been used in, crystallization studies: the full-length protein and a truncated fragment, with a his-tag at the C terminus. Only the second construct resulted in, crystals suitable for x-ray diffraction. The crystals belong to the, monoclinic space group P2(1) with cell dimensions a = 32.20 A, b = 79.57, A, c = 59.21 A, and beta = 94.45, and contain one molecule per asymmetric, unit. At cryogenic temperatures the crystals diffract to a resolution, limit of 2.5 A by using a rotating anode, and beyond 1.8 A by using, synchrotron radiation.
About this StructureAbout this Structure
1FTS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli., Montoya G, Svensson C, Luirink J, Sinning I, Proteins. 1997 Jun;28(2):285-8. PMID:9188744
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