1ffq
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CRYSTAL STRUCTURE OF CHITINASE A COMPLEXED WITH ALLOSAMIDIN
OverviewOverview
The purification scheme of chitinase A (ChiA) from S. marcescens has been, extensively revised. The pure enzyme crystallizes readily under new, crystallization conditions. The ChiA crystal structure has been refined to, 1.55 A resolution and the crystal structure of ChiA co-crystallized with, the inhibitor allosamidin has been refined to 1.9 A resolution., Allosamidin is located in the deep active-site tunnel of ChiA and, interacts with three important residues: Glu315, the proton donor of the, catalysis, Asp313, which adopts two conformations in the native structure, but is oriented towards Glu315 in the inhibitor complex, and Tyr390, which, lies opposite Glu315 in the active-site tunnel.
About this StructureAbout this Structure
1FFQ is a Single protein structure of sequence from Serratia marcescens with AMI as ligand. Active as Chitinase, with EC number 3.2.1.14 Full crystallographic information is available from OCA.
ReferenceReference
De novo purification scheme and crystallization conditions yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin., Papanikolau Y, Tavlas G, Vorgias CE, Petratos K, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):400-3. Epub 2003, Jan 23. PMID:12554965
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