1wgi
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STRUCTURE OF INORGANIC PYROPHOSPHATASE
OverviewOverview
BACKGROUND: Soluble inorganic pyrophosphatase (PPase), an essential enzyme, central to phosphorus metabolism, catalyzes the hydrolysis of the, phosphoanhydride bond in inorganic pyrophosphate. Catalysis requires, divalent metal ions which affect the apparent pKas of the essential, general acid and base on the enzyme, and the pKa of the substrate. Three, to five metal ions are required for maximal activity, depending on pH and, enzyme source. A detailed understanding of catalysis would aid both in, understanding the nature of biological mechanisms of phosphoryl transfer, and in understanding the role of divalent cations. Without a, high-resolution complex structure such a model has previously been, unobtainable. RESULTS: We report the first two high-resolution structures, of yeast PPase, ... [(full description)]
About this StructureAbout this Structure
1WGI is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with MN as [ligand]. Active as [[1]], with EC number [3.6.1.1]. Full crystallographic information is available from [OCA].
ReferenceReference
The structural basis for pyrophosphatase catalysis., Heikinheimo P, Lehtonen J, Baykov A, Lahti R, Cooperman BS, Goldman A, Structure. 1996 Dec 15;4(12):1491-508. PMID:8994974
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