1eno
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BRASSICA NAPUS ENOYL ACP REDUCTASE/NAD BINARY COMPLEX AT PH 8.0 AND ROOM TEMPERATURE
OverviewOverview
BACKGROUND: Enoyl acyl carrier protein reductase (ENR) catalyzes the, NAD(P)H-dependent reduction of trans-delta 2-enoyl acyl carrier protein, an essential step in de novo fatty acid biosynthesis. Plants contain both, NADH-dependent and separate NADPH-dependent ENR enzymes which form part of, the dissociable type II fatty acid synthetase. Highly elevated levels of, the NADH-dependent enzyme are found during lipid deposition in maturing, seeds of oilseed rape (Brassica napus). RESULTS: The crystal structure of, an ENR-NAD binary complex has been determined at 1.9 A resolution and, consists of a homotetramer in which each subunit forms a single domain, comprising a seven-stranded parallel beta sheet flanked by seven alpha, helices. The subunit has a topology highly reminiscent of a, dinucleotide-binding fold. The active site has been located by difference, Fourier analysis of data from crystals equilibrated in NADH. CONCLUSIONS:, The structure of ENR shows a striking similarity with the epimerases and, short-chain alcohol dehydrogenases, in particular, 3 alpha,20, beta-hydroxysteroid dehydrogenase (HSD). The similarity with HSD extends, to the conservation of a catalytically important lysine that stabilizes, the transition state and to the use of a tyrosine as a base--with subtle, modifications arising from differing requirements of the reduction, chemistry.
About this StructureAbout this Structure
1ENO is a Single protein structure of sequence from Brassica napus with NAD as ligand. Active as [acyl-carrier-protein_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number 1.3.1.9 Full crystallographic information is available from OCA.
ReferenceReference
Common themes in redox chemistry emerge from the X-ray structure of oilseed rape (Brassica napus) enoyl acyl carrier protein reductase., Rafferty JB, Simon JW, Baldock C, Artymiuk PJ, Baker PJ, Stuitje AR, Slabas AR, Rice DW, Structure. 1995 Sep 15;3(9):927-38. PMID:8535786 [[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
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