1emy
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CRYSTAL STRUCTURE OF ASIAN ELEPHANT (ELEPHAS MAXIMUS) CYANO-MET MYOGLOBIN AT 1.78 ANGSTROMS RESOLUTION. PHE 29 (B10) ACCOUNTS FOR ITS UNUSUAL LIGAND BINDING PROPERTIES
OverviewOverview
The crystal structure of Asian elephant cyano-metmyoglobin which has a, glutamine instead of the usual distal site histidine has been determined, to high resolution. In addition to this replacement, the substitution of a, conserved leucine residue in position 29(B10) at the distal side by a, phenylalanine was unambiguously identified based on the available electron, density. The suspicion, that there were errors in the original sequence, which has caused some confusion, is thus confirmed. Comparison with other, myoglobin structures in various ligated forms reveals an essentially, unchanged tertiary structure in elephant myoglobin despite the two amino, acid substitutions in the heme pocket. Our current structural model shows, that the N epsilon 2 atom of Gln64(E7) has moved with respect to the, corresponding nitrogen position of His64(E7) in the CO complex of sperm, whale myoglobin. The newly assigned residue Phe29(B10) penetrates into the, distal side of the heme pocket approaching the ligand within van der Waals, distance and causing a much more crowded heme pocket compared to other, myoglobins. Kinetic properties of Asian elephant myoglobin, wild type, and, recombinant sperm whale myoglobins are discussed in relation to the, structural consequences of the two amino acid substitutions H64Q and L29F.
About this StructureAbout this Structure
1EMY is a Single protein structure of sequence from Elephas maximus with CYN and HEM as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Asian elephant (Elephas maximus) cyano-metmyoglobin at 1.78-A resolution. Phe29(B10) accounts for its unusual ligand binding properties., Bisig DA, Di Iorio EE, Diederichs K, Winterhalter KH, Piontek K, J Biol Chem. 1995 Sep 1;270(35):20754-62. PMID:7657658
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