1eg0
FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME
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OverviewOverview
Over 73,000 projections of the E. coli ribosome bound with, formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A, cryo-electron microscopy map of the complex. This map allows, identification of RNA helices, peripheral proteins, and intersubunit, bridges. Comparison of double-stranded RNA regions and positions of, proteins identified in both cryo-EM and X-ray maps indicates good overall, agreement but points to rearrangements of ribosomal components required, for the subunit association. Fitting of known components of the 50S stalk, base region into the map defines the architecture of the GTPase-associated, center and reveals a major change in the orientation of the, alpha-sarcin-ricin loop. Analysis of the bridging connections between the, subunits provides insight into the dynamic signaling mechanism between the, ribosomal subunits.
About this StructureAbout this Structure
1EG0 is a Protein complex structure of sequences from Escherichia coli with 4SU as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of the E. coli 70S ribosome at 11.5 A resolution., Gabashvili IS, Agrawal RK, Spahn CM, Grassucci RA, Svergun DI, Frank J, Penczek P, Cell. 2000 Mar 3;100(5):537-49. PMID:10721991
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