1eep
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2.4 A RESOLUTION CRYSTAL STRUCTURE OF BORRELIA BURGDORFERI INOSINE 5'-MONPHOSPHATE DEHYDROGENASE IN COMPLEX WITH A SULFATE ION
OverviewOverview
The conversion of inosine 5'-monophosphate (IMP) to xanthosine, 5'-monophosphate (XMP) is the committed and rate-limiting reaction in de, novo guanine nucleotide biosynthesis. Inosine 5'- monophosphate, dehydrogenase (IMPDH) is the enzyme that catalyzes the oxidation of IMP to, XMP with the concomitant reduction of nicotinamide adenine dinucleotide, (from NAD(+) to NADH). Because of its critical role in purine, biosynthesis, IMPDH is a drug design target for anticancer, antiinfective, and immunosuppressive chemotherapy. We have determined the crystal, structure of IMPDH from Borrelia burgdorferi, the bacterial spirochete, that causes Lyme disease, with a sulfate ion bound in the IMP phosphate, binding site. This is the first structure of IMPDH in the absence of, substrate or cofactor where the active-site loop (loop 6), which contains, the essential catalytic residue Cys 229, is clearly defined in the, electron density. We report that a seven residue region of loop 6, including Cys229, is tilted more than 6 A away from its position in, substrate- or substrate analogue-bound structures of IMPDH, suggestive of, a conformational change. The location of this loop between beta6 and, alpha6 links IMPDH to a family of beta/alpha barrel enzymes known to, utilize this loop as a functional lid during catalysis. Least-squares, minimization, root-mean-square deviation analysis, and inspection of the, molecular surface of the loop 6 region in the substrate-free B., burgdorferi IMPDH and XMP-bound Chinese hamster IMPDH show that loop 6, follows a similar pattern of hinged rigid-body motion and indicates that, IMPDH may be using loop 6 to bind and sequester substrate and to recruit, an essential catalytic residue.
About this StructureAbout this Structure
1EEP is a Single protein structure of sequence from Borrelia burgdorferi with SO4 as ligand. Active as IMP dehydrogenase, with EC number 1.1.1.205 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure at 2.4 A resolution of Borrelia burgdorferi inosine 5'-monophosphate dehydrogenase: evidence of a substrate-induced hinged-lid motion by loop 6., McMillan FM, Cahoon M, White A, Hedstrom L, Petsko GA, Ringe D, Biochemistry. 2000 Apr 18;39(15):4533-42. PMID:10758003
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