1e4n
CRYSTAL STRUCTURE OF THE INACTIVE MUTANT MONOCOT (MAIZE ZMGLU1) BETA-GLUCOSIDASE ZMGLU E191D IN COMPLEX WITH THE NATURAL AGLYCONE DIMBOA
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OverviewOverview
The mechanism and the site of substrate (i.e., aglycone) recognition and, specificity were investigated in maize beta-glucosidase (Glu1) by x-ray, crystallography by using crystals of a catalytically inactive mutant, (Glu1E191D) in complex with the natural substrate, 2-O-beta-d-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one, (DIMBOAGlc), the free aglycone DIMBOA, and competitive inhibitor, para-hydroxy-S-mandelonitrile beta-glucoside (dhurrin). The structures of, these complexes and of the free enzyme were solved at 2.1-, 2.1-, 2.0-, and 2.2-A resolution, respectively. The structural data from the complexes, allowed us to visualize an intact substrate, free aglycone, or a, competitive inhibitor in the slot-like active site of a beta-glucosidase., These data show that the aglycone moiety of the substrate is sandwiched, between W378 on one side and F198, F205, and F466 on the other. Thus, specific conformations of these four hydrophobic amino acids and the shape, of the aglycone-binding site they form determine aglycone recognition and, substrate specificity in Glu1. In addition to these four residues, A467, interacts with the 7-methoxy group of DIMBOA. All residues but W378 are, variable among beta-glucosidases that differ in substrate specificity, supporting the conclusion that these sites are the basis of aglycone, recognition and binding (i.e., substrate specificity) in, beta-glucosidases. The data also provide a plausible explanation for the, competitive binding of dhurrin to maize beta-glucosidases with high, affinity without being hydrolyzed.
About this StructureAbout this Structure
1E4N is a Single protein structure of sequence from Zea mays with HBO as ligand. Active as Beta-glucosidase, with EC number 3.2.1.21 Full crystallographic information is available from OCA.
ReferenceReference
The mechanism of substrate (aglycone) specificity in beta -glucosidases is revealed by crystal structures of mutant maize beta -glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes., Czjzek M, Cicek M, Zamboni V, Bevan DR, Henrissat B, Esen A, Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13555-60. PMID:11106394
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