1cli
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X-RAY CRYSTAL STRUCTURE OF AMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE (PURM), FROM THE E. COLI PURINE BIOSYNTHETIC PATHWAY, AT 2.5 A RESOLUTION
OverviewOverview
BACKGROUND: The purine biosynthetic pathway in procaryotes enlists eleven, enzymes, six of which use ATP. Enzymes 5 and 6 of this pathway, formylglycinamide ribonucleotide (FGAR) amidotransferase (PurL) and, aminoimidazole ribonucleotide (AIR) synthetase (PurM) utilize ATP to, activate the oxygen of an amide within their substrate toward nucleophilic, attack by a nitrogen. AIR synthetase uses the product of PurL, formylglycinamidine ribonucleotide (FGAM) and ATP to make AIR, ADP and, P(i). RESULTS: The structure of a hexahistidine-tagged PurM has been, solved by multiwavelength anomalous diffraction phasing techniques using, protein containing 28 selenomethionines per asymmetric unit. The final, model of PurM consists of two crystallographically independent dimers and, four sulfates. The overall R factor at 2.5 A resolution is 19.2%, with an, R(free) of 26.4%. The active site, identified in part by conserved, residues, is proposed to be a long groove generated by the interaction of, two monomers. A search of the sequence databases suggests that the, ATP-binding sites between PurM and PurL may be structurally conserved., CONCLUSIONS: The first structure of a new class of ATP-binding enzyme, PurM, has been solved and a model for the active site has been proposed., The structure is unprecedented, with an extensive and unusual, sheet-mediated intersubunit interaction defining the active-site grooves., Sequence searches suggest that two successive enzymes in the purine, biosynthetic pathway, proposed to use similar chemistries, will have, similar ATP-binding domains.
About this StructureAbout this Structure
1CLI is a Single protein structure of sequence from Escherichia coli with SO4 as ligand. Active as Phosphoribosylformylglycinamidine cyclo-ligase, with EC number 6.3.3.1 Full crystallographic information is available from OCA.
ReferenceReference
X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5 A resolution., Li C, Kappock TJ, Stubbe J, Weaver TM, Ealick SE, Structure. 1999 Sep 15;7(9):1155-66. PMID:10508786
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