3c1v
The 1.5 A Crystal structure of Ca2+-bound S100A4
OverviewOverview
S100A4 takes part in control of tumour cell migration and contributes to metastatic spread in in vivo models. In the active dimeric Ca(2+)-bound state it interacts with multiple intracellular targets. Conversely, oligomeric forms of S100A4 are linked with the extracellular function of this protein. We report the 1.5A X-ray crystal structure of Ca(2+)-bound S100A4 and use it to identify the residues involved in target recognition and to derive a model of the oligomeric state. We applied stopped-flow analysis of tyrosine fluorescence to derive kinetics of S100A4 activation by Ca(2+) (k(on)=3.5muM(-1)s(-1), k(off)=20s(-1)).
About this StructureAbout this Structure
3C1V is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the Ca(2+)-form and Ca(2+)-binding kinetics of metastasis-associated protein, S100A4., Gingras AR, Basran J, Prescott A, Kriajevska M, Bagshaw CR, Barsukov IL, FEBS Lett. 2008 May 28;582(12):1651-6. Epub 2008 Apr 22. PMID:18435928 Page seeded by OCA on Wed Jun 4 10:01:51 2008