1bm1
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CRYSTAL STRUCTURE OF BACTERIORHODOPSIN IN THE LIGHT-ADAPTED STATE
OverviewOverview
In the purple membrane of Halobacterium salinarium, bacteriorhodopsin, trimers are arranged in a hexagonal lattice. When purple membrane sheets, are incubated at high temperature with neutral detergent, membrane, vesicularization takes place, yielding inside-out vesicles with a diameter, of 50 nm. The vesicular structure becomes unstable at low temperature, where successive fusion of the vesicles yields a crystal which is composed, of stacked planar membranes. X-ray crystallographic analysis reveals that, the bacteriorhodopsin trimers are arranged in a honeycomb lattice in each, membrane layer and that neighbouring membranes orient in opposite, directions. The native structure of the trimeric unit is preserved in the, honeycomb lattice, irrespective of alterations in the in-plane orientation, of the trimer. One phospholipid tightly bound to a crevice between, monomers in the trimeric unit is suggested to act as a glue in the, formation of the trimer.
About this StructureAbout this Structure
1BM1 is a Single protein structure of sequence from Halobacterium salinarum with RET and DPG as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin., Sato H, Takeda K, Tani K, Hino T, Okada T, Nakasako M, Kamiya N, Kouyama T, Acta Crystallogr D Biol Crystallogr. 1999 Jul;55(Pt 7):1251-6. PMID:10393291
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