1bdx
E. COLI DNA HELICASE RUVA WITH BOUND DNA HOLLIDAY JUNCTION, ALPHA CARBONS AND PHOSPHATE ATOMS ONLY
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OverviewOverview
Here we present the crystal structure of the Escherichia coli protein RuvA, bound to a key DNA intermediate in recombination, the Holliday junction., The structure, solved by isomorphous replacement and density modification, at 6 A resolution, reveals the molecular architecture at the heart of the, branch migration and resolution reactions required to process Holliday, intermediates into recombinant DNA molecules. It also reveals directly for, the first time the structure of the Holliday junction. A single RuvA, tetramer is bound to one face of a junction whose four DNA duplex arms are, arranged in an open and essentially four-fold symmetric conformation., Protein-DNA contacts are mediated by two copies of a helix-hairpin-helix, motif per RuvA subunit that contact the phosphate backbone in a very, similar manner. The open structure of the junction stabilized by RuvA, binding exposes a DNA surface that could be bound by the RuvC endonuclease, to promote resolution.
About this StructureAbout this Structure
1BDX is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of E.coli RuvA with bound DNA Holliday junction at 6 A resolution., Hargreaves D, Rice DW, Sedelnikova SE, Artymiuk PJ, Lloyd RG, Rafferty JB, Nat Struct Biol. 1998 Jun;5(6):441-6. PMID:9628481
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