1qj5

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Revision as of 20:19, 29 October 2007 by OCA (talk | contribs) (New page: left|200px<br /> <applet load="1qj5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qj5, resolution 1.8Å" /> '''CRYSTAL STRUCTURE OF...)
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File:1qj5.gif


1qj5, resolution 1.8Å

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CRYSTAL STRUCTURE OF 7,8-DIAMINOPELARGONIC ACID SYNTHASE

OverviewOverview

The three-dimensional structure of diaminopelargonic acid synthase, a, vitamin B6-dependent enzyme in the pathway of the biosynthesis of biotin, has been determined to 1.8 A resolution by X-ray crystallography. The, structure was solved by multi-wavelength anomalous diffraction techniques, using a crystal derivatized with mercury ions. The protein model has been, refined to a crystallographic R -value of 17.5% (R -free 22.6%). Each, enzyme subunit consists of two domains, a large domain (residues 50-329), containing a seven-stranded predominantly parallel beta-sheet, surrounded, by alpha-helices, and a small domain comprising residues 1-49 and 330-429., Two subunits, related by a non-crystallographic dyad in the crystals, form, the homodimeric molecule, which contains two equal active ... [(full description)]

About this StructureAbout this Structure

1QJ5 is a [Single protein] structure of sequence from [Escherichia coli] with K and PLP as [ligands]. Active as [[1]], with EC number [2.6.1.62]. Full crystallographic information is available from [OCA].

ReferenceReference

Crystal structure of diaminopelargonic acid synthase: evolutionary relationships between pyridoxal-5'-phosphate-dependent enzymes., Kack H, Sandmark J, Gibson K, Schneider G, Lindqvist Y, J Mol Biol. 1999 Aug 27;291(4):857-76. PMID:10452893

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