1av6
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VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE
OverviewOverview
Sequence-nonspecific binding of RNA, recognition of a 7-methylguanosine 5', mRNA cap, and methylation of a nucleic acid backbone are three crucial and, ubiquitous events in eukaryotic nucleic acid processing and function., These three events occur concurrently in the modification of vaccinia, transcripts by the methyltransferase VP39. We report the crystal structure, of a ternary complex comprising VP39, coenzyme product, S-adenosylhomocysteine, and a 5' m7 G-capped, single-stranded RNA hexamer., This structure reveals a novel and general mechanism for, sequence-non-specific recognition of the mRNA transcript in which the, protein interacts solely with the sugar-phosphate backbone of a short, single-stranded RNA helix. This report represents the first direct and, detailed view of a protein complexed with single-stranded RNA or 5'-capped, mRNA.
About this StructureAbout this Structure
1AV6 is a Single protein structure of sequence from Vaccinia virus with MGT and SAH as ligands. Active as Polynucleotide adenylyltransferase, with EC number 2.7.7.19 Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for sequence-nonspecific recognition of 5'-capped mRNA by a cap-modifying enzyme., Hodel AE, Gershon PD, Quiocho FA, Mol Cell. 1998 Feb;1(3):443-7. PMID:9660928
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