2f19
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THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FROM A MONOCLONAL ANTI-ARSONATE ANTIBODY
OverviewOverview
The three-dimensional structure of FabR19.9 from a well-characterized, anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray, diffraction techniques in two crystalline forms (I and II) to a resolution, of 2.8 and 2.7 A, respectively. Essentially the same tertiary and, quaternary structure of the Fab is observed in the two forms. The major, difference resides in the intermolecular contacts, which are interpreted, to favor an irreversible transition from the metastable form I to the more, stable form II. The third complementarity-determining region of the heavy, chain (H3) folds back over the combining site and requires rearrangement, for hapten binding. This dynamic requirement on H3 is consistent with its, mobility in the structure and can explain hapten binding to an otherwise, inaccessible antibody combining site.
About this StructureAbout this Structure
2F19 is a Single protein structure of sequence from [1]. This structure superseeds the now removed PDB entry 1F19. Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional structure of two crystal forms of FabR19.9 from a monoclonal anti-arsonate antibody., Lascombe MB, Alzari PM, Poljak RJ, Nisonoff A, Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9429-33. PMID:1409652
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