1rum
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Crystal structure (F) of H2O2-soaked cationic cyclization antibody 4C6 fab at pH 8.5 with a data set collected at SSRL beamline 9-1.
OverviewOverview
Antibodies can catalyze the generation of hydrogen peroxide (H2O2) from, singlet dioxygen (1O2*) and water via the postulated intermediacy of, dihydrogen trioxide (H2O3) and other trioxygen species. Nine different, crystal structures were determined to elucidate the chemical consequences, to the antibody molecule itself of exposure to such reactive intermediates, and to provide insights into the location on the antibody where these, species could be generated. Herein, we report structural evidence for, modifications of two specific antibody residues within the interfacial, region of the variable and constant domains of different murine antibody, antigen-binding fragments (Fabs) by reactive species generated during the, antibody-catalyzed water oxidation process. Crystal structure analyses of, murine Fabs 4C6 and 13G5 after UV-irradiation revealed complex oxidative, modifications to tryptophan L163 and, in 4C6, hydroxylation of the Cgamma, of glutamine H6. These discrete modifications of specific residues add, further support for the "active site" of the water-oxidation pathway being, located within the interfacial region of the constant and variable domains, and highlight the general resistance of the antibody molecule to oxidation, by reactive oxygen species generated during the water-oxidation process.
About this StructureAbout this Structure
1RUM is a Single protein structure of sequence from Mus musculus with BEZ and GOL as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Probing the antibody-catalyzed water-oxidation pathway at atomic resolution., Zhu X, Wentworth P Jr, Wentworth AD, Eschenmoser A, Lerner RA, Wilson IA, Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2247-52. PMID:14982995
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