2pc6
Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea
OverviewOverview
Crystal structures of two orthologs of the regulatory subunit of acetohydroxyacid synthase III (AHAS, EC 2.2.1.6) from Thermotoga maritima (TM0549) and Nitrosomonas europea (NE1324) were determined by single-wavelength anomalous diffraction methods with the use of selenomethionine derivatives at 2.3 A and 2.5 A, respectively. TM0549 and NE1324 share the same fold, and in both proteins the polypeptide chain contains two separate domains of a similar size. Each protein contains a C-terminal domain with ferredoxin-type fold and an N-terminal ACT domain, of which the latter is characteristic for several proteins involved in amino acid metabolism. The ferredoxin domain is stabilized by a calcium ion in the crystal structure of NE1324 and by a Mg(H2O)(6)2+ ion in TM0549. Both TM0549 and NE1324 form dimeric assemblies in the crystal lattice.
About this StructureAbout this Structure
2PC6 is a Single protein structure of sequence from Nitrosomonas europaea atcc 19718. This structure supersedes the now removed PDB entry 2fgd. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit., Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W, Protein Sci. 2007 Jul;16(7):1360-7. PMID:17586771 Page seeded by OCA on Sun May 4 12:49:25 2008