2iwr
GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)
OverviewOverview
Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.
About this StructureAbout this Structure
2IWR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
The centaurin gamma-1 GTPase-like domain functions as an NTPase., Soundararajan M, Yang X, Elkins JM, Sobott F, Doyle DA, Biochem J. 2007 Feb 1;401(3):679-88. PMID:17037982 Page seeded by OCA on Sun May 4 08:00:02 2008