2qd1
2.2 Angstrom Structure of the human ferrochelatase variant E343K with substrate bound
|
OverviewOverview
Ferrochelatase (protoheme ferrolyase, EC 4.99.1.1) is the terminal enzyme, in heme biosynthesis and catalyzes the insertion of ferrous iron into, protoporphyrin IX to form protoheme IX (heme). Due to the many critical, roles of heme, synthesis of heme is required by the vast majority of, organisms. Despite significant investigation of both the microbial and, eukaryotic enzyme, details of metal chelation remain unidentified. Here we, present the first structure of the wild-type human enzyme, a, lead-inhibited intermediate of the wild-type enzyme with bound metallated, porphyrin macrocycle, the product bound form of the enzyme, and a higher, resolution model for the substrate-bound form of the E343K variant. These, data paint a picture of an enzyme that undergoes significant changes in, secondary structure during the catalytic cycle. The role that these, structural alterations play in overall catalysis and potential, protein-protein interactions with other proteins, as well as the possible, molecular basis for these changes, is discussed. The atomic details and, structural rearrangements presented herein significantly advance our, understanding of the substrate binding mode of ferrochelatase and reveal, new conformational changes in a structurally conserved pi-helix that is, predicted to have a central role in product release.
DiseaseDisease
Known diseases associated with this structure: Protoporphyria, erythropoietic OMIM:[177000], Protoporphyria, erythropoietic, recessive, with liver failure OMIM:[177000]
About this StructureAbout this Structure
2QD1 is a Single protein structure of sequence from Homo sapiens with FES, PP9, CHD and IMD as ligands. Active as Ferrochelatase, with EC number 4.99.1.1 Full crystallographic information is available from OCA.
ReferenceReference
A pi-Helix Switch Selective for Porphyrin Deprotonation and Product Release in Human Ferrochelatase., Medlock AE, Dailey TA, Ross TA, Dailey HA, Lanzilotta WN, J Mol Biol. 2007 Nov 2;373(4):1006-16. Epub 2007 Aug 23. PMID:17884090
Page seeded by OCA on Mon Nov 12 23:32:13 2007