2oj2
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NMR Structure Analysis of the Hematopoetic Cell Kinase SH3 Domain complexed with an artificial high affinity ligand (PD1)
OverviewOverview
We studied the interaction of hematopoietic cell kinase SH3 domain, (HckSH3) with an artificial 12-residue proline-rich peptide PD1, (HSKYPLPPLPSL) identified as high affinity ligand (K(D)=0.2 muM). PD1, shows an unusual ligand sequence for SH3 binding in type I orientation, because it lacks the typical basic anchor residue at position P(-3), but, instead has a tyrosine residue at this position. A basic lysine residue, however, is present at position P(-4). The solution structure of the, HckSH3:PD1 complex, which is the first HckSH3 complex structure available, clearly reveals that the P(-3) tyrosine residue of PD1 does not take the, position of the typical anchor residue but rather forms additional van der, Waals interactions with the HckSH3 RT loop. Instead, lysine at position, P(-4) of PD1 substitutes the function of the P(-3) anchor residue. This, finding expands the well known ligand consensus sequence +xxPpxP by, +xxxPpxP. Thus, software tools like iSPOT fail to identify PD1 as a, high-affinity HckSH3 ligand so far. In addition, a short antiparallel, beta-sheet in the RT loop of HckSH3 is observed upon PD1 binding. The, structure of the HckSH3:PD1 complex reveals novel features of SH3 ligand, binding and yields new insights into the structural basics of SH3-ligand, interactions. Consequences for computational prediction tools adressing, SH3-ligand interactions as well as the biological relevance of our, findings are discussed.
About this StructureAbout this Structure
2OJ2 is a Single protein structure of sequence from Homo sapiens with ACE and NH2 as ligands. This structure superseeds the now removed PDB entry 2A4Y. Active as Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of a Hck SH3 domain ligand complex reveals novel interaction modes., Schmidt H, Hoffmann S, Tran T, Stoldt M, Stangler T, Wiesehan K, Willbold D, J Mol Biol. 2007 Feb 2;365(5):1517-32. Epub 2006 Nov 10. PMID:17141806
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