2io6
Wee1 kinase complexed with inhibitor PD330961
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OverviewOverview
Phosphorylation is critical to regulation of the eukaryotic cell cycle., Entry to mitosis is triggered by the cyclin-dependent kinase CDK1 (Cdc2), which is inactivated during the preceding S and G2 phases by, phosphorylation of T14 and Y15. Two homologous kinases, Wee1, which, phosphorylates Y15, and Myt1, which phosphorylates both T14 and Y15, mediate this inactivation. We have determined the crystal structure of the, catalytic domain of human somatic Wee1 (Wee1A) complexed with an, active-site inhibitor at 1.8 A resolution. Although Wee1A is functionally, a tyrosine kinase, in sequence and structure it most closely resembles, serine/threonine kinases such as Chk1 and cAMP kinases. The crystal, structure shows that although the catalytic site closely resembles that of, other protein kinases, the activation segment contains Wee1-specific, features that maintain it in an active conformation and, together with a, key substitution in its glycine-rich loop, help determine its substrate, specificity.
About this StructureAbout this Structure
2IO6 is a Single protein structure of sequence from Homo sapiens with 330 as ligand. Active as Non-specific protein-tyrosine kinase, with EC number 2.7.10.2 Full crystallographic information is available from OCA.
ReferenceReference
Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation., Squire CJ, Dickson JM, Ivanovic I, Baker EN, Structure. 2005 Apr;13(4):541-50. PMID:15837193
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