2c4u

Revision as of 19:55, 29 October 2007 by OCA (talk | contribs) (New page: left|200px<br /> <applet load="2c4u" size="450" color="white" frame="true" align="right" spinBox="true" caption="2c4u, resolution 2.50Å" /> '''CRYSTAL STRUCTURE O...)
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CRYSTAL STRUCTURE OF THE APO FORM OF THE 5'-FLUORO-5'-DEOXYADENOSINE SYNTHASE ENZYME FROM STREPTOMYCES CATTLEYA

File:2c4u.gif


2c4u, resolution 2.50Å

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OverviewOverview

The fluorinase enzyme from Streptomyces cattleya displays an unusual, ability in biocatalysis in that it forms a C-F bond. We now report that, the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to, accommodate this substrate series. It emerges from this study that the, enzyme does not require a planar ribose conformation of the substrate to, catalyse C-F bond formation.

About this StructureAbout this Structure

2C4U is a [Single protein] structure of sequence from [Streptomyces cattleya] with GOL as [ligand]. Active as [[1]], with EC number [2.5.1.63]. Full crystallographic information is available from [OCA].

ReferenceReference

Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208

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