2gs6
Crystal Structure of the active EGFR kinase domain in complex with an ATP analog-peptide conjugate
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OverviewOverview
The mechanism by which the epidermal growth factor receptor (EGFR) is, activated upon dimerization has eluded definition. We find that the EGFR, kinase domain can be activated by increasing its local concentration or by, mutating a leucine (L834R) in the activation loop, the phosphorylation of, which is not required for activation. This suggests that the kinase domain, is intrinsically autoinhibited, and an intermolecular interaction promotes, its activation. Using further mutational analysis and crystallography we, demonstrate that the autoinhibited conformation of the EGFR kinase domain, resembles that of Src and cyclin-dependent kinases (CDKs). EGFR activation, results from the formation of an asymmetric dimer in which the C-terminal, lobe of one kinase domain plays a role analogous to that of cyclin in, activated CDK/cyclin complexes. The CDK/cyclin-like complex formed by two, kinase domains thus explains the activation of EGFR-family receptors by, homo- or heterodimerization.
DiseaseDisease
Known diseases associated with this structure: Adenocarcinoma of lung, response to tyrosine kinase inhibitor in OMIM:[131550], Nonsmall cell lung cancer, response to tyrosine kinase inhibitor in OMIM:[131550], Nonsmall cell lung cancer, susceptibility to OMIM:[131550]
About this StructureAbout this Structure
2GS6 is a Single protein structure of sequence from Homo sapiens with CL and 112 as ligands. Active as Receptor protein-tyrosine kinase, with EC number 2.7.10.1 Full crystallographic information is available from OCA.
ReferenceReference
An allosteric mechanism for activation of the kinase domain of epidermal growth factor receptor., Zhang X, Gureasko J, Shen K, Cole PA, Kuriyan J, Cell. 2006 Jun 16;125(6):1137-49. PMID:16777603
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