2b8a
High Resolution Structure of the HDGF PWWP Domain
OverviewOverview
Hepatoma Derived Growth Factor (HDGF) is an endogenous nuclear-targeted mitogen that is linked with human disease. HDGF is a member of the weakly conserved PWWP domain family. This 70-amino acid motif, originally identified from the WHSC1 gene, has been found in more than 60 eukaryotic proteins. In addition to the PWWP domain, many proteins in this class contain known chromatin remodeling domains, suggesting a role for HDGF in chromatin remodeling. We have determined the NMR structure of the HDGF PWWP domain to high resolution using a combination of NOEs, J-couplings, and dipolar couplings. Comparison of this structure to a previously determined structure of the HDGF PWWP domain shows a significant difference in the C-terminal region. Comparison to structures of other PWWP domains shows a high degree of similarity to the PWWP domain structures from Dnmt3b and mHRP. The results of selected and amplified binding assay and NMR titrations with DNA suggest that the HDGF PWWP domain may function as a nonspecific DNA-binding domain. Based on the NMR titrations, we propose a model of the interaction of the PWWP domain with DNA.
About this StructureAbout this Structure
2B8A is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
High resolution structure of the HDGF PWWP domain: a potential DNA binding domain., Lukasik SM, Cierpicki T, Borloz M, Grembecka J, Everett A, Bushweller JH, Protein Sci. 2006 Feb;15(2):314-23. Epub 2005 Dec 29. PMID:16384999 Page seeded by OCA on Sat May 3 19:58:34 2008