2itg
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CATALYTIC DOMAIN OF HIV-1 INTEGRASE: ORDERED ACTIVE SITE IN THE F185H CONSTRUCT
OverviewOverview
We solved the structure and traced the complete active site of the, catalytic domain of the human immunodeficiency virus type 1 integrase, (HIV-1 IN) with the F185H mutation. The only previously available crystal, structure, the F185K mutant of this domain, lacks one of the catalytically, important residues, E152, located in a stretch of 12 disordered residues, [Dyda et al. (1994) Science 266, 1981-1986]. It is clear, however, that, the active site of HIV-1 IN observed in either structure cannot correspond, to that of the functional enzyme, since the cluster of three conserved, carboxylic acids does not create a proper metal-binding site. The, conformation of the loop was compared with two different conformations, found in the catalytic domain of the related avian sarcoma virus integrase, ... [(full description)]
About this StructureAbout this Structure
2ITG is a [Single protein] structure of sequence from [Human immunodeficiency virus 1]. Full crystallographic information is available from [OCA].
ReferenceReference
The catalytic domain of human immunodeficiency virus integrase: ordered active site in the F185H mutant., Bujacz G, Alexandratos J, Qing ZL, Clement-Mella C, Wlodawer A, FEBS Lett. 1996 Dec 2;398(2-3):175-8. PMID:8977101
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