2bf8

File:2bf8.gif

spinqualitylabelsall models 2bf8, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K

OverviewOverview

Post-translational modification with small ubiquitin-related modifier, (SUMO) alters the function of many proteins, but the molecular mechanisms, and consequences of this modification are still poorly defined. During a, screen for novel SUMO1 targets, we identified the ubiquitin-conjugating, enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin, thioester and unanchored ubiquitin chain formation in vitro. Crystal, structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate, (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1, interaction through its location on the N-terminal helix. The SUMO, acceptor site in E2-25K, Lys14, does not conform to the consensus site, found in most SUMO targets (PsiKXE), and functions only in the context of, an alpha-helix. In contrast, adjacent SUMO consensus sites are modified, only when in unstructured peptides. The demonstration that secondary, structure elements are part of SUMO attachment signals could contribute to, a better prediction of SUMO targets.

DiseaseDisease

Known diseases associated with this structure: Orofacial cleft 10 OMIM:[601912]

About this StructureAbout this Structure

2BF8 is a Protein complex structure of sequences from Bos taurus and Homo sapiens. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Full crystallographic information is available from OCA.

ReferenceReference

SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079

Page seeded by OCA on Mon Nov 12 21:02:03 2007