2b54
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Human cyclin dependent kinase 2 (CKD2)complexed with DIN-232305
OverviewOverview
Using a high-throughput screening strategy, a series of, 1-aryl-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-4-ones was identified that, inhibit the cyclin-dependent kinase (CDK) 4/cyclin D1 complex-mediated, phosphorylation of a protein substrate with IC(50)s in the low micromolar, range. On the basis of preliminary structure-activity relationships (SAR), a model was proposed in which these inhibitors occupy the ATP-binding site, of the enzyme, forming critical hydrogen bonds to the same residue (Val96), to which the amino group in ATP is presumed to bind. X-ray diffraction, studies on a later derivative bound to CDK2 support this binding mode., Iterative cycles of synthesis and screening lead to a novel series of, potent, CDK2-selective 6-(arylmethyl)pyrazolopyrimidinones. Placement of a, hydrogen-bond donor in the meta-position on the 6-arylmethyl group, resulted in approximately 100-fold increases in CDK4 affinity, giving, ligands that were equipotent inhibitors of CDK4 and CDK2. These compounds, exhibit antiproliferative effects in the NCI HCT116 and other cell lines., The potency of these antiproliferative effects is enhanced in anilide, derivatives and translates into tumor growth inhibition in a mouse, xenograft model.
About this StructureAbout this Structure
2B54 is a Single protein structure of sequence from Homo sapiens with D05 as ligand. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
ReferenceReference
Synthesis and biological evaluation of 1-aryl-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-4-one inhibitors of cyclin-dependent kinases., Markwalder JA, Arnone MR, Benfield PA, Boisclair M, Burton CR, Chang CH, Cox SS, Czerniak PM, Dean CL, Doleniak D, Grafstrom R, Harrison BA, Kaltenbach RF 3rd, Nugiel DA, Rossi KA, Sherk SR, Sisk LM, Stouten P, Trainor GL, Worland P, Seitz SP, J Med Chem. 2004 Nov 18;47(24):5894-911. PMID:15537345
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