2aax
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Mineralocorticoid Receptor Double Mutant with Bound Cortisone
OverviewOverview
Ligand binding is the first step in hormone regulation of, mineralocorticoid receptor (MR) activity. Here, we report multiple crystal, structures of MR (NR3C2) bound to both agonist and antagonists. These, structures combined with mutagenesis studies reveal that maximal receptor, activation involves an intricate ligand-mediated hydrogen bond network, with Asn770 which serves dual roles: stabilization of the loop preceding, the C-terminal activation function-2 helix and direct contact with the, hormone ligand. In addition, most activating ligands hydrogen bond to, Thr945 on helix 10. Structural characterization of the naturally occurring, S810L mutant explains how stabilization of a helix 3/helix 5 interaction, can circumvent the requirement for this hydrogen bond network. Taken, together, these results explain the potency of MR activation by, aldosterone, the weak activation induced by progesterone and the, antihypertensive agent spironolactone, and the binding selectivity of, cortisol over cortisone.
DiseaseDisease
Known diseases associated with this structure: Hypertension, early-onset, autosomal dominant, with exacerbation in pregnancy OMIM:[600983], Pseudohypoaldosteronism type I, autosomal dominant OMIM:[600983]
About this StructureAbout this Structure
2AAX is a Single protein structure of sequence from Homo sapiens with SO4 and PDN as ligands. Full crystallographic information is available from OCA.
ReferenceReference
A ligand-mediated hydrogen bond network required for the activation of the mineralocorticoid receptor., Bledsoe RK, Madauss KP, Holt JA, Apolito CJ, Lambert MH, Pearce KH, Stanley TB, Stewart EL, Trump RP, Willson TM, Williams SP, J Biol Chem. 2005 Sep 2;280(35):31283-93. Epub 2005 Jun 20. PMID:15967794
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