1gxw
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THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE
OverviewOverview
A new crystallization protocol for thermolysin (EC 3.4.24.27) from, Bacillus thermoproteolyticus is presented. After dissolving the protein in, the presence of KSCN, which avoids the use of DMSO and CsCl, crystals were, obtained following the salting-in method. Crystal cell parameters are, isomorphous with those previously reported from DMSO/CsCl mixtures. The, new SCN(-) crystal structure has been analyzed. It shows the presence of, one thiocyanate ion in the catalytic site and several rearrangements in, the S(1) and S(2) subsites. These results are in agreement with the, measurements of Inouye et al. [(1998), J. Biochem. (Tokyo), 123, 847-852], who observed in solution that the solubility of TLN, which is particularly, poor in low ionic strength solutions, increases dramatically in the, ... [(full description)]
About this StructureAbout this Structure
1GXW is a [Single protein] structure of sequence from [Bacillus thermoproteolyticus] with CA, ZN, SCN and VAL as [ligands]. Active as [[1]], with EC number [3.4.24.27]. Full crystallographic information is available from [OCA].
ReferenceReference
The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate., Gaucher JF, Selkti M, Prange T, Tomas A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:12454500
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