1y1h
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human formylglycine generating enzyme, oxidised Cys refined as hydroperoxide
OverviewOverview
Sulfatases are enzymes essential for degradation and remodeling of sulfate, esters. Formylglycine (FGly), the key catalytic residue in the active, site, is unique to sulfatases. In higher eukaryotes, FGly is generated, from a cysteine precursor by the FGly-generating enzyme (FGE). Inactivity, of FGE results in multiple sulfatase deficiency (MSD), a fatal autosomal, recessive syndrome. Based on the crystal structure, we report that FGE is, a single-domain monomer with a surprising paucity of secondary structure, and adopts a unique fold. The effect of all 18 missense mutations found in, MSD patients is explained by the FGE structure, providing a molecular, basis of MSD. The catalytic mechanism of FGly generation was elucidated by, six high-resolution structures of FGE in different redox environments. The, structures allow formulation of a novel oxygenase mechanism whereby FGE, utilizes molecular oxygen to generate FGly via a cysteine sulfenic acid, intermediate.
DiseaseDisease
Known disease associated with this structure: Multiple sulfatase deficiency OMIM:[607939]
About this StructureAbout this Structure
1Y1H is a Single protein structure of sequence from Homo sapiens with SR and PEO as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Molecular basis for multiple sulfatase deficiency and mechanism for formylglycine generation of the human formylglycine-generating enzyme., Dierks T, Dickmanns A, Preusser-Kunze A, Schmidt B, Mariappan M, von Figura K, Ficner R, Rudolph MG, Cell. 2005 May 20;121(4):541-52. PMID:15907468
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