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Human mitochondrial ribosome in complex with mRNA, A-site tRNA, P-site tRNA and E-site tRNAHuman mitochondrial ribosome in complex with mRNA, A-site tRNA, P-site tRNA and E-site tRNA
Structural highlights
FunctionPublication Abstract from PubMedTranslation of mitochondrial messenger RNA (mt-mRNA) is performed by distinct mitoribosomes comprising at least 36 mitochondria-specific proteins. How these mitoribosomal proteins assist in the binding of mt-mRNA and to what extent they are involved in the translocation of transfer RNA (mt-tRNA) is unclear. To visualize the process of translation in human mitochondria, we report ~3.0 A resolution structure of the human mitoribosome, including the L7/L12 stalk, and eight structures of its functional complexes with mt-mRNA, mt-tRNAs, recycling factor and additional trans factors. The study reveals a transacting protein module LRPPRC-SLIRP that delivers mt-mRNA to the mitoribosomal small subunit through a dedicated platform formed by the mitochondria-specific protein mS39. Mitoribosomal proteins of the large subunit mL40, mL48, and mL64 coordinate translocation of mt-tRNA. The comparison between those structures shows dynamic interactions between the mitoribosome and its ligands, suggesting a sequential mechanism of conformational changes. Structural basis of mitochondrial translation.,Aibara S, Singh V, Modelska A, Amunts A Elife. 2020 Aug 19;9. pii: 58362. doi: 10.7554/eLife.58362. PMID:32812867[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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