2n4k

Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31

Structural highlights 2n4k is a 1 chain structure with sequence from Enterococcus faecium. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 20 models Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ETCHF_ENTFC Bacteriocin.[REFERENCE:2] Publication Abstract from PubMed The solution structure of enterocin HF (EntHF), a class IIa bacteriocin of 43 amino acids produced by Enterococcus faecium M3K31, was evaluated by CD and NMR spectroscopy. Purified EntHF was unstructured in water, but CD analysis supports that EntHF adopts an alpha-helical conformation when exposed to increasing concentrations of trifluoroethanol. Furthermore, NMR spectroscopy indicates that this bacteriocin adopts an antiparallel beta-sheet structure in the N-terminal region (residues 1-17), followed by a well-defined central alpha-helix (residues 19-30) and a more disordered C-terminal end (residues 31-43). EntHF could be structurally organized into three flexible regions that might act in a coordinated manner. This is in agreement with the absence of long-range nuclear Overhauser effect signals between the beta-sheet domain and the C-terminal end of the bacteriocin. The 3D structure recorded for EntHF fits emerging facts regarding target recognition and mode of action of class IIa bacteriocins. Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31.,Arbulu S, Lohans CT, van Belkum MJ, Cintas LM, Herranz C, Vederas JC, Hernandez PE J Agric Food Chem. 2015 Dec 16;63(49):10689-95. doi: 10.1021/acs.jafc.5b03882., Epub 2015 Dec 1. PMID:26585399 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 2n4k Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image