4uds
Crystal structure of MbdR regulator from Azoarcus sp. CIBCrystal structure of MbdR regulator from Azoarcus sp. CIB
Structural highlights
Publication Abstract from PubMedThe mbd cluster encodes the anaerobic degradation of 3-methylbenzoate in the beta-proteobacterium Azoarcus sp. CIB. The specific transcriptional regulation circuit that controls the expression of the mbd genes was investigated. The PO, PB1, and P3R promoters responsible for the expression of the mbd genes, their cognate MbdR transcriptional repressor, as well as the MbdR operator regions (ATAC-N10-GTAT), have been characterized. The three-dimensional structure of MbdR has been solved revealing a conformation similar to that of other TetR family transcriptional regulators. The first intermediate of the catabolic pathway, i.e., 3-methylbenzoyl-CoA, was shown to act as the inducer molecule. An additional MbdR-dependent promoter, PA, which contributes to the expression of the CoA ligase that activates 3-methylbenzoate to 3-methylbenzoyl-CoA, was shown to be necessary for an efficient induction of the mbd genes. Our results suggest that the mbd cluster recruited a regulatory system based on the MbdR regulator and its target promoters, to evolve a distinct central catabolic pathway that is only expressed for the anaerobic degradation of aromatic compounds that generate 3-methylbenzoyl-CoA as central metabolite. All these results highlight the importance of the regulatory systems in the evolution and adaptation of bacteria to the anaerobic degradation of aromatic compounds. Unraveling the Specific Regulation of the mbd Central Pathway for the Anaerobic Degradation of 3-methylbenzoate.,Juarez JF, Liu H, Zamarro MT, McMahon S, Liu H, Naismith JH, Eberlein C, Boll M, Carmona M, Diaz E J Biol Chem. 2015 Mar 20. pii: jbc.M115.637074. PMID:25795774[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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