1csg
Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factorThree-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor
Structural highlights
FunctionCSF2_HUMAN Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four alpha-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel beta-sheet. The tertiary structure of rhGM-CSF has a topology similar to that of porcine growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus. Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor.,Walter MR, Cook WJ, Ealick SE, Nagabhushan TL, Trotta PP, Bugg CE J Mol Biol. 1992 Apr 20;224(4):1075-85. PMID:1569568[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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