9cc8

Hexameric state of the NRC4 resistosomeHexameric state of the NRC4 resistosome

Structural highlights 9cc8 is a 6 chain structure with sequence from Nicotiana benthamiana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.66Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A5J6DCT7_NICBE Publication Abstract from PubMed Innate immune responses to microbial pathogens are regulated by intracellular receptors known as nucleotide-binding leucine-rich repeat receptors (NLRs) in both the plant and animal kingdoms. Across plant innate immune systems, "helper" NLRs (hNLRs) work in coordination with "sensor" NLRs (sNLRs) to modulate disease resistance signaling pathways. Activation mechanisms of hNLRs based on structures are unknown. Our research reveals that the hNLR, known as NLR required for cell death 4 (NRC4), assembles into a hexameric resistosome upon activation by the sNLR Bs2 and the pathogenic effector AvrBs2. This conformational change triggers immune responses by facilitating the influx of calcium ions (Ca(2+)) into the cytosol. The activation mimic alleles of NRC2, NRC3, or NRC4 alone did not induce Ca(2+) influx and cell death in animal cells, suggesting that unknown plant-specific factors regulate NRCs' activation in plants. These findings significantly advance our understanding of the regulatory mechanisms governing plant immune responses. Activation of the helper NRC4 immune receptor forms a hexameric resistosome.,Liu F, Yang Z, Wang C, You Z, Martin R, Qiao W, Huang J, Jacob P, Dangl JL, Carette JE, Luan S, Nogales E, Staskawicz BJ Cell. 2024 Sep 5;187(18):4877-4889.e15. doi: 10.1016/j.cell.2024.07.013. Epub , 2024 Aug 1. PMID:39094568 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 9cc8, resolution 2.66Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image